ImmunoPheno: a data-driven platform for the design and analysis of immunophenotyping experiments

ImmunoPheno is a data-driven bioinformatics platform designed to facilitate the design and analysis of immunophenotyping experiments. It addresses the limitations of current cytometry techniques by enabling researchers to design optimized antibody panels for specific cell populations and to more accurately identify and annotate cells in multiplexed antibody-based cytometry data. By leveraging single-cell proteotranscriptomic atlases (e.g., CITE-seq), ImmunoPheno enhances the phenotypic resolution, annotation, accuracy, and reproducibility in immunophenotyping workflows.

Contents

OVERVIEW

• What is ImmunoPheno?

TUTORIALS

• Tutorial 1: Finding Antibodies for Gating a Specific Cell Population
• Tutorial 2: Normalizing CITE-seq data
• Tutorial 3: Automated Annotation of Cell Identities in mIHC Data
• Tutorial 4: Design of Antibody Panels

GALAXY USAGE

• Galaxy Platform: Normalizing CITE-Seq Data
• Galaxy Platform: Automated Annotation of Cell Identities in mIHC Data
• Galaxy Platform: Design of Antibody Panels

API

• Normalizing CITE-Seq Data
  • Installation
  • ImmunoPhenoData
• Querying the Database
  • ImmunoPhenoDB_Connect
• Plots
  • Plotting Functions